Department: EMB – Embryology
Salary: Based on experience
Location: Baltimore, MD
The Ludington Lab seeks an able and motivated career technician to manage our gnotobiotic fruit fly work and maintain organization of the lab and be a part of the lab ethos. Candidates should be able to design and carry out laboratory experiments, working independently and effectively while managing and prioritizing multiple projects. This position requires a thorough understanding and vigilant implementation of the scientific method, which will be required to accomplish assigned projects.
- Maintain germ-free and gnotobiotic fly stocks for the lab.
- Derive germ-free flies and inoculate them with specific bacteria (which makes the gnotobiotic).
- Keep track of media supplies and coordinate orders for the lab to maintain ample supplies. This will require daily to weekly coordination with the part-time media technician.
- Fly breeding, sort unmated females, perform defined crosses, genotyping.
- Fly phenotyping, such as lifespan, fecundity, development time, automated behavior assays, dissecting gut tissues and preparing them for downstream analyses including fixing and staining for microscopy.
- Perform basic molecular biology experiments such as, but not limited to, molecular cloning, PCR, making competent cells, bacterial transformation, DNA miniprep, DNA plasmid prep, restriction enzyme digest, running and analyzing agarose gel data to determine results.
- Basic microbiology culture, including freeze cells, pull cells from -80˚C freezer stocks, culture cells on agar or liquid media, measure OD, plate to count CFUs, perform dilution series.
- Use a liquid handling robot to make defined combinations of bacterial strains to inoculate flies.
- Maintain and monitor shared resources, i.e. lab stocks such as plasmids, antibodies, and chemicals.
- Make general shared lab stock solutions, including making aliquots and store them in an organized manner.
- Perform literature searches to find necessary information for projects, e.g. recipes and protocols.
- Attend and participate in lab meetings.
- Work efficiently and cooperatively with other researchers in support of lab goals and projects.
- Organize and lead lab supply purchases
- Maintain the cleanliness of common lab space
- Participate in rotation based lab duty.
- Maintain precise records of work.
- Other duties as assigned.
Please note: training will be provided as needed
Bachelor degree in relevant science area and two years relevant lab experience – Or Master degree and one year relevant lab experience.
Knowledge and practice of basic laboratory biology techniques, and ability to learn, adapt, and carry out established written protocols independently.
Strong organizational and computer skills, meticulous attention to detail.
Excellent verbal and written communication skills
Enthusiasm for developing new skills and expertise as needed to achieve lab goals.
Ability to work independently and effectively.
The Department of Embryology is one of six departments comprising the Carnegie Institution for Science. The department functions as a private, non-profit research institute affiliated with the Johns Hopkins University and is located adjacent to the JHU Homewood campus in Baltimore, MD. The department consists of 10 laboratories studying a variety of questions in cell and developmental biology. For more information about the Department, visit https://emb.carnegiescience.edu/
Carnegie is an equal opportunity employer. All qualified applicants will receive consideration for employment and will not be discriminated against on the basis of gender, race/ethnicity, protected veteran status, disability, or other protected group status.
Please submit a copy of your resume or CV with the application.