Department: EMB – Embryology
Salary: Based on experience
Location: Baltimore, MD
The Kostova laboratory at Carnegie’s Department of Embryology in Baltimore is searching for a full-time research technician. This is a great opportunity to work directly with a principal investigator using cutting edge technologies to answer fundamental questions of cell biology. Ideal candidates should have a B.S. in Biology or related field, experience in modern molecular biology techniques, and a strong work ethic.
We are investigating the fundamental question of how cells respond when their ribosomes break down. The ribosome is one the most complex and long-lived molecular machines in the cell and the presence of functional ribosomes is essential to live. Ribosome damage has been recently linked to complex human diseases, such as cancer and neurodegeneration. We use cutting-edge technology, such as CRISPR-mediated genome editing and genome scale screens, to model and map the molecular pathways that ensure ribosome integrity.
We are looking for a highly motivated individual with strong organizational skills to join our team and help explore these novel areas of biology.
- Grow and clone mammalian cell lines (tissue culture)
- Perform CRISPR-mediated genome editing and characterize the obtained mammalian cell lines (genotyping, qPCR, Western blotting, FACS analysis)
- Perform high-throughput genetic screens and prepare libraries for Illumina sequencing
- Organize and maintain lab databases (plasmids, oligos, reagents, cell lines, etc.)
- S. in Biology (or equivalent)
- 1-2 years of lab experience
- Strong interpersonal skills and ability to work as part of a team
- Mammalian tissue culture
- Modern molecular biology techniques, e.g., DNA/RNA purification, cDNA synthesis, qPCR, plasmid cloning, SDSPAGE, and Western blotting
- Great organizational skills
- Excellent written and oral communication skills
The Department of Embryology is one of six departments comprising the Carnegie Institution for Science. The department functions as a private, non-profit research institute affiliated with the Johns Hopkins University and is located adjacent to the JHU Homewood campus in Baltimore, MD. The department consists of 10 laboratories studying a variety of questions in cell and developmental biology. For more information about the Department, visit https://emb.carnegiescience.edu/
Carnegie is an equal opportunity employer. All qualified applicants will receive consideration for employment and will not be discriminated against on the basis of gender, race/ethnicity, protected veteran status, disability, or other protected group status.