Job: Senior Laboratory Technician – Bortvin Lab

Department: EMB – Embryology

Salary: TBD

Location: Baltimore,MD

Position Overview

The Bortvin laboratory at the Carnegie Institution’s Embryology Department (https://emb.carnegiescience.edu/), located on the Johns Hopkins University Homewood campus in Baltimore, MD, seeks an enthusiastic and motivated Senior Research Technician. This is a full-time position with competitive salary and benefits, with an anticipated start date in October 1, 2020.

Essential Job Duties:

  • Applying standard and advanced molecular biology methods to generate expression vectors in bacterial and mammalian cells
  • Performing flow cytometry analysis and purification of mouse embryonic stem cells and germ cells
  • Mouse husbandry, genetic crosses, and dissection of embryonic and adult tissues
  • Performing laser confocal fluorescence microscopy including super-resolution microscopy
  • Performing nucleic acid purification and analysis including next generation sequencing approaches
  • Developing and optimizing protocols for analysis of DNA modifications
  • Derivation, culture and genetic modification of mouse embryonic stem cells including CRISPR/Cas9.
  • Analyzing and interpreting experimental data
  • Working with Dr. Bortvin on preparing manuscripts describing research findings
  • Meeting routinely with Dr. Bortvin to discuss research progress
  • Attending weekly laboratory meetings and preparing regular oral presentations
  • Maintaining precise records of work, in both hand-written and electronic formats
  • Assisting with support duties relating to general lab management and organization
  • Maintaining professional conduct that supports a cooperative, collegial and inclusive research environment.

Desired Qualifications:

Applicants must hold a Master’s degree in life sciences or related discipline, with at least one year of post-bac experience working in a life sciences research laboratory. Hands-on experience with molecular biological methods, CRISPR/Cas9-based genome engineering, recombinant protein production are required. Strong analytical, communication and organization skills are a must.

To be considered for this position, please send the following materials to https://jobs.carnegiescience.edu/jobs/emb/:

  • Brief cover letter including long-term career goals
  • Résumé emphasizing prior research experience
  • Contact information for three references
  • Undergraduate and graduate transcripts (unofficial copies are fine)
  • Publications (if available).

Priority will be given to candidates who apply by September 15, 2020.

Additional information:

The Department of Embryology is one of six departments comprising the Carnegie Institution for Science. The department functions as a private, non-profit research institute affiliated with the Johns Hopkins University and is located adjacent to the JHU Homewood campus in Baltimore, MD. The department consists of 10 laboratories studying a variety of questions in cell and developmental biology. For more information about the Department, visit https://emb.carnegiescience.edu/Carnegie is an equal opportunity employer. All qualified applicants will receive consideration for employment and will not be discriminated against on the basis of gender, race/ethnicity, protected veteran status, disability, or other protected group status.