Department: EMB – Embryology
The Bortvin laboratory at the Carnegie Institution’s Embryology Department (https://emb.carnegiescience.edu/), located on the Johns Hopkins University Homewood campus in Baltimore, MD, seeks an enthusiastic and motivated Senior Research Technician. This is a full-time position with competitive salary and benefits, with an anticipated start date in October 1, 2020.
Essential Job Duties:
- Applying standard and advanced molecular biology methods to generate expression vectors in bacterial and mammalian cells
- Performing flow cytometry analysis and purification of mouse embryonic stem cells and germ cells
- Mouse husbandry, genetic crosses, and dissection of embryonic and adult tissues
- Performing laser confocal fluorescence microscopy including super-resolution microscopy
- Performing nucleic acid purification and analysis including next generation sequencing approaches
- Developing and optimizing protocols for analysis of DNA modifications
- Derivation, culture and genetic modification of mouse embryonic stem cells including CRISPR/Cas9.
- Analyzing and interpreting experimental data
- Working with Dr. Bortvin on preparing manuscripts describing research findings
- Meeting routinely with Dr. Bortvin to discuss research progress
- Attending weekly laboratory meetings and preparing regular oral presentations
- Maintaining precise records of work, in both hand-written and electronic formats
- Assisting with support duties relating to general lab management and organization
- Maintaining professional conduct that supports a cooperative, collegial and inclusive research environment.
Applicants must hold a Master’s degree in life sciences or related discipline, with at least one year of post-bac experience working in a life sciences research laboratory. Hands-on experience with molecular biological methods, CRISPR/Cas9-based genome engineering, recombinant protein production are required. Strong analytical, communication and organization skills are a must.
To be considered for this position, please send the following materials to https://jobs.carnegiescience.edu/jobs/emb/:
- Brief cover letter including long-term career goals
- Résumé emphasizing prior research experience
- Contact information for three references
- Undergraduate and graduate transcripts (unofficial copies are fine)
- Publications (if available).
Priority will be given to candidates who apply by September 15, 2020.
The Department of Embryology is one of six departments comprising the Carnegie Institution for Science. The department functions as a private, non-profit research institute affiliated with the Johns Hopkins University and is located adjacent to the JHU Homewood campus in Baltimore, MD. The department consists of 10 laboratories studying a variety of questions in cell and developmental biology. For more information about the Department, visit https://emb.carnegiescience.edu/. Carnegie is an equal opportunity employer. All qualified applicants will receive consideration for employment and will not be discriminated against on the basis of gender, race/ethnicity, protected veteran status, disability, or other protected group status.