EMB – Embryology

Postdoctoral Fellow Cleves & Moi Lab

Department: EMB – Embryology

Salary: TBD

Location: Baltimore, MD

Overview:

The Cleves Lab and The Moi Lab at the Carnegie Institution’s Department of Embryology and Plant Biology seek an ambitious postdoctoral fellow interested in using genome-wide association approaches and CRISPR/Cas9 methods to identify and functionally test natural alleles associated with ecologically important traits in coral species. They will drive a collaborative project working closely with the Cleves lab and the Moi Lab. Specifically, the laboratory work of this postdoctoral position will be based in the Cleves lab at the Carnegie Institution’s Department of Embryology in Baltimore, MD, with remote computational work to be conducted in close collaboration with the Moi lab at the Carnegie’s Department of Plant Biology at Stanford, CA.

The Cleves Lab uses genetic, biochemical, and cell biology methods to understand the molecular and cellular bases of coral-algal symbiosis and why it breaks down due to stress. We use a variety of organisms from corals in the field to tractable model organisms in the lab (e.g., the small anemone, Aiptasia). Recently, we have developed CRISPR/Cas9 knock-out technology in reef-building corals offering an exciting opportunity to understand coral gene function for the first time. In addition to these genetic tools, we use a combination of single-cell RNAseq, genomics, proteomics, and state-of-the-art microscopy to understand the cell biology of these charismatic and ecologically important organisms.

The Moi Lab is focused on understanding the genetic basis of ecologically adaptive traits. Using statistical and evolutionary genetic approaches, we infer how natural selection by climate have shaped species genetic makeup and geographic distributions. Both of which are key to understanding species responses to climate change.

Position Details:

We seek a postdoc interested in applying modern genome-wide methods and advancing current CRISPR/Cas9 methods to identify and functionally test genomic regions of natural selection in coral populations. Because of the nature of the research, the ideal candidate would have experience in both genome-wide association studies and  in using genetic editing approaches in model or non-model organisms with an interest in cell and developmental biology. This position is currently fully funded for two years as a collaborative effort between the two Carnegie Labs.

Relevant papers:

  1. Cleves PA, Krediet CJ, Lehnert EM, Tinoco AI, Onishi M, Pringle JR. Insights into the mechanisms of coral bleaching from analysis of gene expression during heat stress in a sea-anemone model system. Proc Natl Acad Sci USA. 117(46): 28906-28917
  1. Cleves PA, Tinoco AI, Bradford J, Perrin D, Bay LK, Pringle JR. Reduced thermal tolerance in a coral carrying CRISPR-induced mutations in the gene for a heat-shock transcription factor. Proc Natl Acad Sci USA. 117(46): 28899-28905
  1. Cleves PA, Shumaker A, Lee J, Putnam HM, Bhattacharya D.Unknown to known: advancing knowledge of coral gene function. Trends Genet. 36(2):93-104
  1. Cleves PA, Strader ME, Bay LK, Pringle JR, Matz MV. CRISPR/Cas9-mediated genome editing in a reef-building coral. Proc Natl Acad Sci USA. 115(20): 5235-5240
  1. Capblancq, T., M. C. Fitzpatrick, R. A. Bay, M. Exposito-Alonso, and S. R. Keller. Genomic Prediction of (Mal)Adaptation Across Current and Future Climatic Landscapes. Annual review of ecology, evolution, and systematics 51:245–269.
  1. Exposito-Alonso, M., 500 Genomes Field Experiment Team, H. A. Burbano, O. Bossdorf, R. Nielsen, and D. Weigel. Natural selection in the Arabidopsis thaliana genome in present and future climates. Nature 573:126–129.
  1. Exposito-Alonso, M., F. Vasseur, W. Ding, G. Wang, H. A. Burbano, and D. Weigel. Genomic basis and evolutionary potential for extreme drought adaptation in Arabidopsis thaliana. Nature ecology & evolution 2:352–358.

Please click below to apply. Applications must include:

  1. A cover letter highlighting past research and includes a proposed direction of post-doc research in our lab.
  2.  CV
  3.  A list of three references.

Informal inquiries and questions should be directed to Dr. Phillip Cleves at cleves@carnegiescience.edu

About the research environment:

The Department of Embryology is one of six departments comprising the Carnegie Institution for Science. The department functions as a private, non-profit research institute affiliated with the Johns Hopkins University and is located adjacent to the JHU Homewood campus in Baltimore, MD. The Department consists of 10 laboratories studying a variety of questions in cell and developmental biology. For more information about the Department, visit https://emb.carnegiescience.edu/.

Additional Information:

Carnegie is an equal opportunity employer. All applicants will receive consideration of employment without regard to race, color, religion, gender, sexual orientation, gender identity or expression, national origin, age, genetic information, disability, veteran status, or any other characteristics protected by law.

At Carnegie, we are committed to building a diverse and inclusive community. We believe academic environments should be places where diverse groups of people with various viewpoints and ideas can thrive and work together. As such, we encourage applicants from under-represented groups and backgrounds to apply.

 

 

 

 

 

Research Technician

Department: EMB – Embryology

Salary: TBD

Location: Baltimore, MD

Job Summary:

The Kostova laboratory at Carnegie’s Department of Embryology in Baltimore is searching for a full-time research technician. This is a great opportunity to work with a postdoctoral fellow using cutting-edge technologies to answer fundamental questions of cell biology. Ideal candidates should have a B.S. in Biology or a related field, experience in modern molecular biology techniques, a strong work ethic, and be able to join the position for at least 2 years.

We are investigating the fundamental question of how different types of ribosomes regulate translation and embryogenesis. Despite ribosomes’ importance, they are traditionally seen with minimal regulatory activity. Exciting new studies challenge this concept and provide evidence that organisms assemble ribosomes with different makeups during development in response to stress, including the development of multiple cancers. Although ribosome heterogeneity is hypothesized to alter translation, little experimental data exist to demonstrate this. The zebrafish embryo provides a power system to tackle this question. We use cutting-edge technology, such as genome editing and high throughput sequencing technology, to tag different ribosome populations and determine their ability to synthesize proteins.

We are looking for a highly motivated individual with strong organizational skills to join our team and help explore these novel areas of biology.

Key Responsibilities:

  • Perform genome editing and characterize zebrafish strains (genotyping, qPCR, Western blotting)
  • Isolation of ribosomes and RNA analysis (sucrose gradient fractionation, immunoprecipitation, RNA purification, and electrophoresis)
  • Maintenance and upkeep of zebrafish-related infrastructure

Requirements:

  • B.S. in Biology (or equivalent)
  • Lab experience
  • Strong interpersonal skills and ability to work as part of a team

Preferred Skills:

  • Modern molecular biology techniques, e.g., DNA/RNA purification, cDNA synthesis, qPCR, plasmid cloning, SDS­PAGE, and Western blotting
  • Some experience working with zebrafish or other vertebrates in a research laboratory.
  • Great organizational skills
  • Excellent written and oral communication skills

If interested please send the following documents to kostova@carnegiescience.edu:

  • Cover letter
  • Curriculum Vitae
  • Academic transcript
  • Recommendation letters (at least 2)

Research Environment:

The Department of Embryology is one of six departments comprising the Carnegie Institution for Science. The department functions as a private, non-profit research institute affiliated with Johns Hopkins University and is located adjacent to the JHU Homewood campus in Baltimore, MD. The Department consists of 10 laboratories studying a variety of questions in cell and developmental biology. For more information about the Department, visit https://emb.carnegiescience.edu/.

Additional Information:

Carnegie is an equal opportunity employer. All applicants will receive consideration for employment without regard to race, color, religion, gender, sexual orientation, gender identity or expression, national origin, age, genetic information, disability, veteran status, or any other characteristics protected by law.

At Carnegie, we are committed to building a diverse and inclusive community. We believe academic environments should be places where diverse groups of people with various viewpoints and ideas can thrive and work together. As such, we encourage applicants from under-represented groups and backgrounds to apply.

 

Research Associate & Lab Manager

Department: EMB – Embryology

Salary: TBD

Location: Baltimore, MD

The Belin lab (http://www.belinlab.org) is a research group at the Carnegie Institution’s Embryology Department (https://emb.carnegiescience.edu/), located on the Johns Hopkins University Homewood campus in Baltimore, MD. We seek one research scientist and one lab manager to facilitate our research on the molecular mechanisms of environmental host-microbe interactions. Both positions are full-time with a competitive salary and benefits packages and flexible start dates.

The selected candidates will be joining a small, highly collegial laboratory within a large and vibrant research community, with many opportunities to present their work in departments throughout Johns Hopkins and Carnegie. Belin lab members have access to facilities and equipment across both institutes, including a suite of newly installed growth chambers, mass spec, and state-of-the-art super-resolution microscopes. Dr. Belin is committed to the professional development of all hires, with opportunities available to participate in grant writing, mentorship, and science communication activities. Depending upon funding availability, hires also may have opportunities to attend and present their research at scientific conferences.

Research Associate Qualifications and Duties:

Applicants must have a M.S. degree or higher in the life sciences or a related discipline with at least 1 year of full-time postgraduate research experience, or a B.S. degree with at least 3 years of full-time post-graduate experience. Expertise in plant cultivation, small molecule analysis, quantitative microscopy, or bacterial genetics in non-model bacteria is desirable. Applicants must have excellent organizational skills and proficiency with record-keeping and scientific presentations.

Core job duties will include bench and computational work on both independent projects, in addition to providing research support for other lab members as needed. Candidates may be expected to change their research focus based on the labs’ current funding and research priorities, at the discretion of Dr. Belin. Additional responsibilities include, but are not limited to:

  • Preparing research results for publications or presentations
  • Contributing to the preparation of research grants
  • Training and mentoring junior research scientists
  • Coordinating collaborations with external research groups
  • Contributing to duties described below for the Lab Manager position when assistance is required
  • Committing to ensuring a safe and inclusive lab environment

Lab Manager Qualifications and Duties:

Applicants must have a B.S. degree in the life sciences or a related discipline and exceptional organizational and communication skills. Candidates also must be reliable and available to work within standard 9 am – 5 pm weekday hours. Specific responsibilities will include, but are not limited to:

  • Sterilizing glassware, consumables, and reagents
  • Preparing common lab stocks including bacterial plates and buffers
  • Ordering and stocking lab supplies and equipment
  • Performing basic equipment maintenance, managing service contracts, and coordinating technician visits
  • Coordinating with other Embryology lab managers and department personnel on the management of shared department facilities
  • Maintaining lab databases including online protocols, bacterial strain and plasmid libraries, and seed stocks
  • Long-term cultivation of plants in grow tents and growth chambers
  • Helping Dr. Belin to organize social activities for the lab and lab guests
  • Working with Dr. Belin to develop and ensure compliance with lab safety protocols and policies
  • Committing to ensuring a safe and inclusive lab environment

Experience in some of the above activities is highly desirable. Limited work on research projects may be possible, depending on the background and interest of the candidate.

How to Apply:

To be considered, please send the following materials to belin at carnegiescience dot edu :

  • Brief cover letter including long-term career goals
  • CV emphasizing prior research experience
  • Contact information for three references

 

Research Associate applicants should also include:

  • Publications or preprints (if available)

Additional information:

The Department of Embryology is one of several departments and divisions comprising the Carnegie Institution for Science. The department functions as a private, non-profit research institute affiliated with the Johns Hopkins University and is located adjacent to the JHU Homewood campus in Baltimore, MD. The department consists of 10 laboratories studying a variety of questions in cell and developmental biology. For more information about the Department, visit https://emb.carnegiescience.edu/

Carnegie is an equal opportunity employer. All qualified applicants will receive consideration for employment and will not be discriminated against on the basis of gender, race/ethnicity, protected veteran status, disability, or other protected group status. 

Postdoctoral Fellow

Department: EMB – Embryology

Salary: TBD

Location: Baltimore, MD

Overview:

The Zheng Lab at the Carnegie Institution’s Department of Embryology seeks ambitious postdoctoral fellows interested in understanding the role of lamins in mouse development. The Zheng lab has developed mouse models that allow the study of individual and different combinations of the three lamin genes using germline or conditional lamin knockout. The lab also developed mouse embryonic stem cells lacking all or different combinations of lamins. By employing a wide range of genomic tools and cell biological approaches, the lab has observed a number of interesting phenotypes in different lamin knockout combinations. We seek a postdoctoral fellow with skills in analyzing mouse early embryos, ES cell derivation, and the use of ES cells to study differentiation.

Selected publications:

  1. Kim Y…Zheng Y (2011). Mouse ES cells do not need any lamins but proper organogenesis requires lamin-Bs. Science 334:1706-1710.
  1. Jia J…Zheng Y (2012). Regulation of pluripotency and self-renewal of ES cells through epigenetic threshold modulation and mRNA pruning. Cell 151:576–589.
  1. Chen H, Chen X, and Zheng Y (2013). The nuclear lamina regulates germline stem cell niche organization via modulation of EGFR signaling. Cell Stem Cell 13:73-86.
  2. Chen H, Zheng X, and Zheng Y (2014). Age-associated loss of lamin-B leads to systemic inflammation and gut hyperplasia. Cell 159:829-843. Zheng X…Zheng Y (2018). Lamins organize the global three-dimensional genome from the nuclear periphery. Molecular Cell DOI: https://doi.org/10.1016/j.molcel.2018.05.017 .
Additional Information:

The Department of Embryology is one of six departments comprising the Carnegie Institution for Science. The department functions as a private, non-profit research institute affiliated with the Johns Hopkins University and is located adjacent to the JHU Homewood campus in Baltimore, MD. The department consists of 10 laboratories studying a variety of questions in cell and developmental biology. For more information about the Department, visit https://emb.carnegiescience.edu/

Carnegie is an equal opportunity employer. All qualified applicants will receive consideration for employment and will not be discriminated against on the basis of gender, race/ethnicity, protected veteran status, disability, or other protected group status.

Please submit a copy of your resume or CV with the application.  If you have further questions please reach out to Dr. Yixian Zheng, Principal Investigator.

 

 

Postdoctoral Associate

Department: EMB – Embryology

Salary: NIH-NRSA Stipend Scale

Location: Baltimore, MD

Description:

Postdoctoral Associates carry out research that explores the current frontiers of knowledge in basic biological science under the guidance of a Carnegie faculty member. A list of faculty, their areas of current research, and additional information is available at: emb.carnegiescience.edu.

They conceive, carry out and publish novel discoveries based on their research that contribute to human understanding of biological mechanisms and present these findings to the national and international scientific community.

Qualifications:

PhD degree and record of distinguished research accomplishments in a relevant discipline as documented by first-author publications, presentation of research at scientific meetings and letters of recommendation from leading scientists.

Additional information:

The Department of Embryology is one of six departments comprising the Carnegie Institution for Science. The department functions as a private, non-profit research institute affiliated with the Johns Hopkins University and is located adjacent to the JHU Homewood campus in Baltimore, MD. The department consists of 10 laboratories studying a variety of questions in cell and developmental biology. For more information about the Department, visit https://emb.carnegiescience.edu/

Carnegie is an equal opportunity employer. All qualified applicants will receive consideration for employment and will not be discriminated against on the basis of gender, race/ethnicity, protected veteran status, disability, or other protected group status.

Research Technician- Kostova Lab

Department: EMB – Embryology

Salary: Based on experience

Location: Baltimore, MD

Job Summary:

The Kostova laboratory at Carnegie’s Department of Embryology in Baltimore is searching for a full-time research technician. This is a great opportunity to work directly with a principal investigator using cutting edge technologies to answer fundamental questions of cell biology. Ideal candidates should have a B.S. in Biology or related field, experience in modern molecular biology techniques, and a strong work ethic.

We are investigating the fundamental question of how cells respond when their ribosomes break down. The ribosome is one the most complex and long-lived molecular machines in the cell and the presence of functional ribosomes is essential to live. Ribosome damage has been recently linked to complex human diseases, such as cancer and neurodegeneration. We use cutting-edge technology, such as CRISPR-mediated genome editing and genome scale screens, to model and map the molecular pathways that ensure ribosome integrity.

We are looking for a highly motivated individual with strong organizational skills to join our team and help explore these novel areas of biology.

Key Responsibilities:

  • Grow and clone mammalian cell lines (tissue culture)
  • Perform CRISPR-mediated genome editing and characterize the obtained mammalian cell lines (genotyping, qPCR, Western blotting, FACS analysis)
  • Perform high-throughput genetic screens and prepare libraries for Illumina sequencing
  • Organize and maintain lab databases (plasmids, oligos, reagents, cell lines, etc.)

Requirements:

  • S. in Biology (or equivalent)
  • 1-2 years of lab experience
  • Strong interpersonal skills and ability to work as part of a team

Preferred Skills:

  • Mammalian tissue culture
  • Modern molecular biology techniques, e.g., DNA/RNA purification, cDNA synthesis, qPCR, plasmid cloning, SDS­PAGE, and Western blotting
  • Great organizational skills
  • Excellent written and oral communication skills

Additional information:

The Department of Embryology is one of six departments comprising the Carnegie Institution for Science. The department functions as a private, non-profit research institute affiliated with the Johns Hopkins University and is located adjacent to the JHU Homewood campus in Baltimore, MD. The department consists of 10 laboratories studying a variety of questions in cell and developmental biology. For more information about the Department, visit https://emb.carnegiescience.edu/

Carnegie is an equal opportunity employer. All qualified applicants will receive consideration for employment and will not be discriminated against on the basis of gender, race/ethnicity, protected veteran status, disability, or other protected group status.