EMB – Embryology

Senior Research Technician – Bortvin Lab

Department: EMB – Embryology

Salary: TBD

Location: Baltimore,MD

Position Overview

The Bortvin laboratory at the Carnegie Institution’s Embryology Department (https://emb.carnegiescience.edu/), located on the Johns Hopkins University Homewood campus in Baltimore, MD, seeks an enthusiastic and motivated Senior Research Technician. This is a full-time position with competitive salary and benefits, with an anticipated start date in October 1, 2020.

Essential Job Duties:

  • Applying standard and advanced molecular biology methods to generate expression vectors in bacterial and mammalian cells
  • Performing flow cytometry analysis and purification of mouse embryonic stem cells and germ cells
  • Mouse husbandry, genetic crosses, and dissection of embryonic and adult tissues
  • Performing laser confocal fluorescence microscopy including super-resolution microscopy
  • Performing nucleic acid purification and analysis including next generation sequencing approaches
  • Developing and optimizing protocols for analysis of DNA modifications
  • Derivation, culture and genetic modification of mouse embryonic stem cells including CRISPR/Cas9.
  • Analyzing and interpreting experimental data
  • Working with Dr. Bortvin on preparing manuscripts describing research findings
  • Meeting routinely with Dr. Bortvin to discuss research progress
  • Attending weekly laboratory meetings and preparing regular oral presentations
  • Maintaining precise records of work, in both hand-written and electronic formats
  • Assisting with support duties relating to general lab management and organization
  • Maintaining professional conduct that supports a cooperative, collegial and inclusive research environment.

Desired Qualifications:

Applicants must hold a Master’s degree in life sciences or related discipline, with at least one year of post-bac experience working in a life sciences research laboratory. Hands-on experience with molecular biological methods, CRISPR/Cas9-based genome engineering, recombinant protein production are required. Strong analytical, communication and organization skills are a must.

To Apply:

To be considered for this position, please send the following materials to https://jobs.carnegiescience.edu/jobs/emb/:

  • Brief cover letter including long-term career goals
  • Résumé emphasizing prior research experience
  • Contact information for three references
  • Undergraduate and graduate transcripts (unofficial copies are acceptable)
  • Publications (if available)

Priority will be given to candidates who apply by September 15, 2020.

Additional information:

The Department of Embryology is one of six departments comprising the Carnegie Institution for Science. The department functions as a private, non-profit research institute affiliated with the Johns Hopkins University and is located adjacent to the JHU Homewood campus in Baltimore, MD. The department consists of 10 laboratories studying a variety of questions in cell and developmental biology. For more information about the Department, visit https://emb.carnegiescience.edu/.

 

At Carnegie, we are committed to building a diverse and inclusive community. We believe academic environments should be places where diverse groups of people with a variety of viewpoints and ideas can thrive and work together. As such, we encourage applicants from under-represented groups and backgrounds to apply.

The Carnegie Institution is an equal opportunity employer. All qualified applicants will receive consideration of employment without regard to race, color, religion, gender, sexual orientation, gender identity or expression, national origin, age, genetic information, disability, veteran status or any other protected group status.

Electron Microscopy Specialist

Department: EMB – Embryology

Salary: TBD

Location: Baltimore, MD

Carnegie Institution for Science has an exciting opportunity for an Electron Microscopy Specialist working at our Embryology Department located on the campus of Johns Hopkins University. The ideal candidate will a motivated individual with strong electron microscopy (EM) expertise (>3 years) to run and maintain our core EM facility with a newly acquired Hitachi HT 7800 (with an AMT Nanosprint 12 camera running Hitachi software) and equipment for histology and sectioning.

Principal Responsibilities:

The most important responsibility of this position is to provide electron microscopy service to all labs who need it. This involves acquisition and fixation of specimens, embedding, cutting and photography of samples as well as instruction on any phase of the process that the lab may choose. Routine upkeep and maintenance of the EM and all other laboratory equipment. It also includes providing histology and cryoscopy services and instruction to all labs.

Other Duties:

Employees at our department help with various common tasks beyond their main job responsibilities. This position will have the following additional and infrequent duties:

Stocking and distributing supplies:

  • Order and maintain the common stockroom to ensure adequate supplies are maintained for the department and maintain the database to ensure proper billing for the labs and department.
  • Provide ethanol for the labs as part of the stockroom and maintain records according to state and federal regulations.
  • Maintain liquid nitrogen stocks for lab usage.
  • Stocking and distributing needles per department needs.

Safety:

  • Provide laboratory safety training to new hires as well as informing the labs as to proper safety procedures according to our safety manual. Arrange proper disposal of hazardous material.
  • Provide radiation safety instruction to new hires and labs as well as proper radiation safety procedures according to our safety manual. Maintain proper radiation records according to both state and federal regulations. Upkeep of the radiation lab and equipment. Arrange proper disposal of radioactive material.
  • Provide other duty or service needed for the operation of the facility when needed.

Education, Experience and Skills:

  • Bachelors degree in biological science required
  • Certification from the Microscopy Society of America strongly preferred
  • Electron microscopy experience required; strong computer skills
  • Strong work ethic and reliable
  • Demonstrated attention to detail
  • Effective communication and time management skills
  • Able to work both independently and as part of a team

Physical Requirements:

  • Ability to transport and/or move objects weighing up to 25 pounds
  • Ability to stoop, bend, reach and grasp with arms and hands above head
  • Able to perform periods of standing and walking around the building to perform the responsibilities
  • Willing and able to handle laboratory waste materials according to mandated procedures required
  • Willing and able to handle potentially hazardous chemicals, low level radioactive materials, and biological materials using mandated safety protocols and procedures is required

The Embryology Department is one of five departments comprising the Carnegie Institution for Science. Carnegie is a non-profit research institute and our department is affiliated with the Johns Hopkins University (JHU) located on the JHU Homewood campus in Baltimore, MD. We are home to 10 laboratories, studying a variety of questions in molecular, cell and developmental biology.

To Apply:  Interested individuals should submit a cover letter, full CV, and the names and contact information of at least three references to gilmer@carnegiescience.edu. Carnegie offers excellent employee benefits. To learn more, visit us here.

At Carnegie, we are committed to building a diverse and inclusive community. We believe academic environments should be places where diverse groups of people with a variety of viewpoints and ideas can thrive and work together. As such, we encourage applicants from under-represented groups and backgrounds to apply.

The Carnegie Institution is an equal opportunity employer. All qualified applicants will receive consideration of employment without regard to race, color, religion, gender, sexual orientation, gender identity or expression, national origin, age, genetic information, disability, veteran status or any other protected group status.

 

 

 

Lab Technician- Entry Level

Department: EMB – Embryology

Salary: TBD

Location: Baltimore, MD

Laboratory Technician

Location: Baltimore, MD

Overview:

The Fan Lab of the Carnegie Institution for Science, Department of Embryology is seeking an enthusiastic and organized Laboratory Technician. Please click here for additional information about the Fan Lab. Those with an interest in bio-medical research are strongly encouraged to apply.

Desired Qualifications:

B.S. in Biology

Strong attention to detail

Ability to work independently and in a group setting

Additional Information:

The Department of Embryology is one of five departments comprising the Carnegie Institution for Science. The department functions as a private, non-profit research institute affiliated with the Johns Hopkins University and is located adjacent to the JHU Homewood campus in Baltimore, MD. The department consists of 10 laboratories studying a variety of questions in cell and developmental biology. For more information about the Department, visit https://emb.carnegiescience.edu/.

At Carnegie, we are committed to building a diverse and inclusive community. We believe academic environments should be places where diverse groups of people with a variety of viewpoints and ideas can thrive and work together. As such, we encourage applicants from under-represented groups and backgrounds to apply.

The Carnegie Institution is an equal opportunity employer. All qualified applicants will receive consideration of employment without regard to race, color, religion, gender, sexual orientation, gender identity or expression, national origin, age, genetic information, disability, veteran status or any other protected group status.

 

Postdoctoral Fellow

Department: EMB – Embryology

Salary: TBD

Location: Baltimore, MD

Overview:

The Zheng Lab at the Carnegie Institution’s Department of Embryology seeks ambitious postdoctoral fellows interested in understanding the role of lamins in mouse development. The Zheng lab has developed mouse models that allow the study of individual and different combinations of the three lamin genes using germline or conditional lamin knockout. The lab also developed mouse embryonic stem cells lacking all or different combinations of lamins. By employing a wide range of genomic tools and cell biological approaches, the lab has observed a number of interesting phenotypes in different lamin knockout combinations. We seek a postdoctoral fellow with skills in analyzing mouse early embryos, ES cell derivation, and the use of ES cells to study differentiation.

Selected publications:

  1. Kim Y…Zheng Y (2011). Mouse ES cells do not need any lamins but proper organogenesis requires lamin-Bs. Science 334:1706-1710.
  1. Jia J…Zheng Y (2012). Regulation of pluripotency and self-renewal of ES cells through epigenetic threshold modulation and mRNA pruning. Cell 151:576–589.
  1. Chen H, Chen X, and Zheng Y (2013). The nuclear lamina regulates germline stem cell niche organization via modulation of EGFR signaling. Cell Stem Cell 13:73-86.
  2. Chen H, Zheng X, and Zheng Y (2014). Age-associated loss of lamin-B leads to systemic inflammation and gut hyperplasia. Cell 159:829-843. Zheng X…Zheng Y (2018). Lamins organize the global three-dimensional genome from the nuclear periphery. Molecular Cell DOI: https://doi.org/10.1016/j.molcel.2018.05.017 .
Additional Information:

The Department of Embryology is one of six departments comprising the Carnegie Institution for Science. The department functions as a private, non-profit research institute affiliated with the Johns Hopkins University and is located adjacent to the JHU Homewood campus in Baltimore, MD. The department consists of 10 laboratories studying a variety of questions in cell and developmental biology. For more information about the Department, visit https://emb.carnegiescience.edu/

Carnegie is an equal opportunity employer. All qualified applicants will receive consideration for employment and will not be discriminated against on the basis of gender, race/ethnicity, protected veteran status, disability, or other protected group status.

Please submit a copy of your resume or CV with the application.  If you have further questions please reach out to Dr. Yixian Zheng, Principal Investigator.

 

 

Postdoctoral Fellow-Kostova Lab

Department: EMB – Embryology

Salary: TBD

Location: Baltimore, MD

Overview: A postdoctoral position is available in the laboratory of Dr. Kamena Kostova (https://kostovalab.org/) at the Carnegie Institution for Science, Department of Embryology in Baltimore, MD. We are investigating the fundamental question of how cells respond when their ribosomes break down. The ribosome is one the most complex and long-lived molecular machines in the cell and the presence of functional ribosomes is essential to live. Ribosome damage has been recently linked to complex human diseases, such as cancer and neurodegeneration. We use cutting-edge technology, such as CRISPR-Cas9-mediated genome editing and genome scale screens, to model and map the molecular pathways that ensure ribosome integrity in mammalian cell lines and zebrafish.

Qualifications: An ideal candidate will be creative, industrious, have excellent communication skills, and enjoy working independently as well as collaboratively. A Ph.D. in a related field and fluency in spoken and written English is required for the position.

To Apply: Please send a curriculum vitae, statement of research interest, and contact information for three references to kostova@carnegiescience.edu.

Additional Information:

The Department of Embryology is one of five departments comprising the Carnegie Institution for Science. The department functions as a private, non-profit research institute affiliated with the Johns Hopkins University and is located adjacent to the JHU Homewood campus in Baltimore, MD. We are home to 10 laboratories studying a variety of questions in cell and developmental biology. For more information about the Department, visit http://emb.carnegiescience.edu/

At Carnegie, we are committed to building a diverse and inclusive community. We believe academic environments should be places where diverse groups of people with a variety of viewpoints and ideas can thrive and work together. As such, we encourage applicants from under-represented groups and backgrounds to apply.

The Carnegie Institution is an equal opportunity employer. All qualified applicants will receive consideration of employment without regard to race, color, religion, gender, sexual orientation, gender identity or expression, national origin, age, genetic information, disability, veteran status or any other protected group status.