Department: EMB – Embryology
Salary: NIH-NRSA Postdoctoral Stipend Scale
Location: Baltimore, MD
The Zheng Lab at the Department of Embryology seeks ambitious postdoctoral fellows interested in pursuing one of the following two areas of research listed below.
The role of genome organization in development, tissue function, and aging.
How cells in different lineages acquire and maintain their unique genome architecture has remained poorly understood. We use various tools and organisms to study how genome organization in different cells influences lineage specification and terminal differentiation, how such organization is maintained in differentiated cells, and whether genome dis-organization accompanies aging and contributes to organ decay.
- Kim Y…Zheng Y (2011). Mouse ES cells do not need any lamins but proper organogenesis requires lamin-Bs. Science 334:1706-1710.
- Jia J…Zheng Y (2012). Regulation of pluripotency and self-renewal of ES cells through epigenetic threshold modulation and mRNA pruning. Cell 151:576–589.
- Chen H, Chen X, and Zheng Y (2013). The nuclear lamina regulates germline stem cell niche organization via modulation of EGFR signaling. Cell Stem Cell 13:73-86.
- Chen H, Zheng X, and Zheng Y (2014). Age-associated loss of lamin-B leads to systemic inflammation and gut hyperplasia. Cell 159:829-843. Zheng X…Zheng Y (2018). Lamins organize the global three-dimensional genome from the nuclear periphery. Molecular Cell DOI: https://doi.org/10.1016/j.molcel.2018.05.017 .
Cell division mechanisms
We are interested in understanding how cells segregate chromosomes equally into daughter cells using mitotic spindle apparatus. We discovered protein complexes called γ-tubulinring complex (γTuRC) and γ-tubulin small complex (γTuSC) that mediate microtubule nucleation and organization in mitotic and interphase cells. By using the powerful Xenopus egg extract system, we and others have uncovered an important signaling pathway mediated by the nuclear small GTPase Ran that regulates multiple aspects of cell division. More recently we discovered a new spindle assembly factor called BuGZ that regulates mitosis in part by its ability to undergo protein phase separation or transition.
- Zheng Y…Mitchison TJ (1995). A γ-tubulin ring complex from the unfertilized eggof Xenopus laevis can nucleate microtubule assembly in vitro. Nature 378:578-583.
- Wilde A & Zheng Y (1999). Stimulation of microtubule aster formation and spindle assembly in Xenopus egg extracts by the small GTPase Ran. Science 284:1359-1362.
- Wiese C…Zheng Y (2001). Role of importin-b coupling Ran to downstream targets in microtubule assembly. Science 291:653-656.
- Tsai M-Y…Zheng Y (2006). A mitotic lamin B matrix induced by RanGTP required for spindle assembly. Science 311:1887-1893.
- Jiang H…Zheng Y (2014). A microtubule-associate zinc finger protein, BuGZ, regulated mitotic chromosome alignment by ensuring Bub3 stability and kinetochore targeting. Developmental Cell 28:268-281.
- Jiang H…Zheng Y (2015). Phase transition of spindle-associated protein regulate spindle apparatus assembly. Cell 163:108-122.
The Department of Embryology is one of six departments comprising the Carnegie Institution for Science. The department functions as a private, non-profit research institute affiliated with the Johns Hopkins University and is located adjacent to the JHU Homewood campus in Baltimore, MD. The department consists of 10 laboratories studying a variety of questions in cell and developmental biology. For more information about the Department, visit https://emb.carnegiescience.edu/
Carnegie is an equal opportunity employer. All qualified applicants will receive consideration for employment and will not be discriminated against on the basis of gender, race/ethnicity, protected veteran status, disability, or other protected group status.
Please submit a copy of your resume or CV with the application. If you have further questions please reach out to Dr. Yixian Zheng, Principal Investigator.