EMB – Embryology

Postdoctoral Fellow Cleves & Moi Lab

Department: EMB – Embryology

Salary: TBD

Location: Baltimore, MD

Overview:

The Cleves Lab and The Moi Lab at the Carnegie Institution’s Department of Embryology and Plant Biology seek an ambitious postdoctoral fellow interested in using genome-wide association approaches and CRISPR/Cas9 methods to identify and functionally test natural alleles associated with ecologically important traits in coral species. They will drive a collaborative project working closely with the Cleves lab and the Moi Lab. Specifically, the laboratory work of this postdoctoral position will be based in the Cleves lab at the Carnegie Institution’s Department of Embryology in Baltimore, MD, with remote computational work to be conducted in close collaboration with the Moi lab at the Carnegie’s Department of Plant Biology at Stanford, CA.

The Cleves Lab uses genetic, biochemical, and cell biology methods to understand the molecular and cellular bases of coral-algal symbiosis and why it breaks down due to stress. We use a variety of organisms from corals in the field to tractable model organisms in the lab (e.g., the small anemone, Aiptasia). Recently, we have developed CRISPR/Cas9 knock-out technology in reef-building corals offering an exciting opportunity to understand coral gene function for the first time. In addition to these genetic tools, we use a combination of single-cell RNAseq, genomics, proteomics, and state-of-the-art microscopy to understand the cell biology of these charismatic and ecologically important organisms.

The Moi Lab is focused on understanding the genetic basis of ecologically adaptive traits. Using statistical and evolutionary genetic approaches, we infer how natural selection by climate have shaped species genetic makeup and geographic distributions. Both of which are key to understanding species responses to climate change.

Position Details:

We seek a postdoc interested in applying modern genome-wide methods and advancing current CRISPR/Cas9 methods to identify and functionally test genomic regions of natural selection in coral populations. Because of the nature of the research, the ideal candidate would have experience in both genome-wide association studies and  in using genetic editing approaches in model or non-model organisms with an interest in cell and developmental biology. This position is currently fully funded for two years as a collaborative effort between the two Carnegie Labs.

Relevant papers:

  1. Cleves PA, Krediet CJ, Lehnert EM, Tinoco AI, Onishi M, Pringle JR. Insights into the mechanisms of coral bleaching from analysis of gene expression during heat stress in a sea-anemone model system. Proc Natl Acad Sci USA. 117(46): 28906-28917
  1. Cleves PA, Tinoco AI, Bradford J, Perrin D, Bay LK, Pringle JR. Reduced thermal tolerance in a coral carrying CRISPR-induced mutations in the gene for a heat-shock transcription factor. Proc Natl Acad Sci USA. 117(46): 28899-28905
  1. Cleves PA, Shumaker A, Lee J, Putnam HM, Bhattacharya D.Unknown to known: advancing knowledge of coral gene function. Trends Genet. 36(2):93-104
  1. Cleves PA, Strader ME, Bay LK, Pringle JR, Matz MV. CRISPR/Cas9-mediated genome editing in a reef-building coral. Proc Natl Acad Sci USA. 115(20): 5235-5240
  1. Capblancq, T., M. C. Fitzpatrick, R. A. Bay, M. Exposito-Alonso, and S. R. Keller. Genomic Prediction of (Mal)Adaptation Across Current and Future Climatic Landscapes. Annual review of ecology, evolution, and systematics 51:245–269.
  1. Exposito-Alonso, M., 500 Genomes Field Experiment Team, H. A. Burbano, O. Bossdorf, R. Nielsen, and D. Weigel. Natural selection in the Arabidopsis thaliana genome in present and future climates. Nature 573:126–129.
  1. Exposito-Alonso, M., F. Vasseur, W. Ding, G. Wang, H. A. Burbano, and D. Weigel. Genomic basis and evolutionary potential for extreme drought adaptation in Arabidopsis thaliana. Nature ecology & evolution 2:352–358.

Please click below to apply. Applications must include:

  1. A cover letter highlighting past research and includes a proposed direction of post-doc research in our lab.
  2.  CV
  3.  A list of three references.

Informal inquiries and questions should be directed to Dr. Phillip Cleves at cleves@carnegiescience.edu

About the research environment:

The Department of Embryology is one of six departments comprising the Carnegie Institution for Science. The department functions as a private, non-profit research institute affiliated with the Johns Hopkins University and is located adjacent to the JHU Homewood campus in Baltimore, MD. The Department consists of 10 laboratories studying a variety of questions in cell and developmental biology. For more information about the Department, visit https://emb.carnegiescience.edu/.

Additional Information:

Carnegie is an equal opportunity employer. All applicants will receive consideration of employment without regard to race, color, religion, gender, sexual orientation, gender identity or expression, national origin, age, genetic information, disability, veteran status, or any other characteristics protected by law.

At Carnegie, we are committed to building a diverse and inclusive community. We believe academic environments should be places where diverse groups of people with various viewpoints and ideas can thrive and work together. As such, we encourage applicants from under-represented groups and backgrounds to apply.

 

 

 

 

 

Bioinformatics Associate/Postdoctoral

Department: EMB – Embryology

Salary: TBD

Location: Baltimore, MD

Department: EMB – Embryology

Salary: Commensurate with experience

Location: Baltimore, MD

Description

The Department of Embryology at the Carnegie Institution seeks an organized individual with excellent communication skills to join our Bioinformatics team.  This successful applicant will collaborate with a team of genomics and bioinformatics specialists to provide analysis support and training to 10 research labs applying next-generation sequencing and single cell analysis to study fundamental mechanisms in coral biology, genome organization, lipid metabolism, molecular ecology, muscle regeneration, and stem cell biology.

The successful candidate will work with members of the genomics and bioinformatics team to support researchers using a mix of existing Unix workflows and custom analysis with R/Bioconductor.  Emphasis will be placed on conducting reproducible analyses and creating comprehensible reports.

Equally important is the training of our scientists in the latest data science methodologies through a combination of in person and online training.  Our partnerships with Johns Hopkins University, Loyola University Maryland, and Clovis Community College provide several opportunities to reach larger communities and help train the next generation of biologists.

Qualifications

– MS in Bioinformatics or PhD in Biology with bioinformatics experience

– Excellent communication and task management skills

– Proficiency with R/RStudio and Bioconductor

– Basic experience with reproducible research tools (e.g. Git, Notebooks)

– Empathy teaching computational skills to biologists new to data analysis

– Experience creating sequencing libraries and using single-cell technologies is desired

How to Apply

  1. Cover letter including brief description of a bioinformatics collaboration or experience teaching bioinformatics
  2. CV, including a link to GitHub repository if available
  3. Examples of an analysis report and teaching material if available
  4. Contact information for three references

Additional Information

Carnegie is an equal opportunity employer. All qualified applicants will receive consideration for employment and will not be discriminated against on the basis of gender, race/ethnicity, protected veteran status, disability, or other protected group status.

At Carnegie, we are committed to building a diverse and inclusive community. We believe academic environments should be places where diverse groups of people with various viewpoints and ideas can thrive and work together. As such, we encourage applicants from under-represented groups and backgrounds to apply.

The Department of Embryology is one of six departments comprising the Carnegie Institution for Science. The department functions as a private, non-profit research institute affiliated with the Johns Hopkins University and is located adjacent to the JHU Homewood campus in Baltimore, MD. The Department consists of 10 laboratories studying a variety of questions in cell and developmental biology, with a new emphasis on symbiosis. For more information about the Department, visit https://emb.carnegiescience.edu.

Postdoc/Senior Research Scientist

Department: EMB – Embryology

Salary:

Location: Baltimore, MD

Position: Postdoc/Senior Research Scientist

Department: EMB

Lab: Belin Lab

Overview:

The Belin lab (http://www.belinlab.org) is a newly established research group at the Carnegie Institution’s Embryology Department (https://emb.carnegiescience.edu/), located on the Johns Hopkins University Homewood campus in Baltimore, MD. We seek motivated postdocs or senior research scientists to study the cell biology of nitrogen-fixing bacteria and their symbioses with plants. Two projects are currently available, relating to the roles of microbially-produced plant hormones in plant-microbe symbioses and the functions of lipids in bacterial membrane organization; candidates may also develop new projects based on their own research interests. Both positions are full-time with competitive salary and benefit packages and flexible start dates.

The selected candidates will be joining a small, highly collegial laboratory within a large and vibrant research community, with many opportunities to present their work to multiple departments throughout Johns Hopkins and Carnegie. Belin lab members have access to facilities and equipment across both institutes, including a suite of newly installed plant growth chambers and auxiliary growth room, mass spec, and state-of-the-art super-resolution confocal microscopes intended for use during these projects. Dr. Belin is committed to the professional development of all hires, with opportunities available to participate in grant writing, mentorship of junior lab members, and lab management. Postdoc candidates additionally will receive ample support in the preparation of fellowship proposals and applications for independent research positions and will be provided with a formal mentorship committee of senior faculty.

Qualifications:

Applicants must have a Ph.D. in the life sciences or a related discipline. While expertise in plant cultivation, small molecule characterization, super resolution microscopy, or bacterial genetics are desirable, highly curious candidates from any area of the life sciences who are enthusiastic about learning new skills are encouraged to apply.

How to Apply:

To be considered, please send the following materials to belin at carnegiescience dot edu :

  • Brief cover letter including long-term career goals
  • CV emphasizing prior research experience
  • Contact information for three references
  • Publications or preprints (if available)

Additional information:

The Department of Embryology is one of six departments comprising the Carnegie Institution for Science. The department functions as a private, non-profit research institute affiliated with the Johns Hopkins University and is located adjacent to the JHU Homewood campus in Baltimore, MD. The department consists of 10 laboratories studying a variety of questions in cell and developmental biology. For more information about the Department, visit https://emb.carnegiescience.edu/

Carnegie is an equal opportunity employer. All qualified applicants will receive consideration for employment and will not be discriminated against on the basis of gender, race/ethnicity, protected veteran status, disability, or other protected group status. 

Postdoctoral Fellow

Department: EMB – Embryology

Salary: TBD

Location: Baltimore, MD

Overview:

The Zheng Lab at the Carnegie Institution’s Department of Embryology seeks ambitious postdoctoral fellows interested in understanding the role of lamins in mouse development. The Zheng lab has developed mouse models that allow the study of individual and different combinations of the three lamin genes using germline or conditional lamin knockout. The lab also developed mouse embryonic stem cells lacking all or different combinations of lamins. By employing a wide range of genomic tools and cell biological approaches, the lab has observed a number of interesting phenotypes in different lamin knockout combinations. We seek a postdoctoral fellow with skills in analyzing mouse early embryos, ES cell derivation, and the use of ES cells to study differentiation.

Selected publications:

  1. Kim Y…Zheng Y (2011). Mouse ES cells do not need any lamins but proper organogenesis requires lamin-Bs. Science 334:1706-1710.
  1. Jia J…Zheng Y (2012). Regulation of pluripotency and self-renewal of ES cells through epigenetic threshold modulation and mRNA pruning. Cell 151:576–589.
  1. Chen H, Chen X, and Zheng Y (2013). The nuclear lamina regulates germline stem cell niche organization via modulation of EGFR signaling. Cell Stem Cell 13:73-86.
  2. Chen H, Zheng X, and Zheng Y (2014). Age-associated loss of lamin-B leads to systemic inflammation and gut hyperplasia. Cell 159:829-843. Zheng X…Zheng Y (2018). Lamins organize the global three-dimensional genome from the nuclear periphery. Molecular Cell DOI: https://doi.org/10.1016/j.molcel.2018.05.017 .
Additional Information:

The Department of Embryology is one of six departments comprising the Carnegie Institution for Science. The department functions as a private, non-profit research institute affiliated with the Johns Hopkins University and is located adjacent to the JHU Homewood campus in Baltimore, MD. The department consists of 10 laboratories studying a variety of questions in cell and developmental biology. For more information about the Department, visit https://emb.carnegiescience.edu/

Carnegie is an equal opportunity employer. All qualified applicants will receive consideration for employment and will not be discriminated against on the basis of gender, race/ethnicity, protected veteran status, disability, or other protected group status.

Please submit a copy of your resume or CV with the application.  If you have further questions please reach out to Dr. Yixian Zheng, Principal Investigator.

 

 

Postdoctoral Associate

Department: EMB – Embryology

Salary: NIH-NRSA Stipend Scale

Location: Baltimore, MD

Description:

Postdoctoral Associates carry out research that explores the current frontiers of knowledge in basic biological science under the guidance of a Carnegie faculty member. A list of faculty, their areas of current research, and additional information is available at: emb.carnegiescience.edu.

They conceive, carry out and publish novel discoveries based on their research that contribute to human understanding of biological mechanisms and present these findings to the national and international scientific community.

Qualifications:

PhD degree and record of distinguished research accomplishments in a relevant discipline as documented by first-author publications, presentation of research at scientific meetings and letters of recommendation from leading scientists.

Additional information:

The Department of Embryology is one of six departments comprising the Carnegie Institution for Science. The department functions as a private, non-profit research institute affiliated with the Johns Hopkins University and is located adjacent to the JHU Homewood campus in Baltimore, MD. The department consists of 10 laboratories studying a variety of questions in cell and developmental biology. For more information about the Department, visit https://emb.carnegiescience.edu/

Carnegie is an equal opportunity employer. All qualified applicants will receive consideration for employment and will not be discriminated against on the basis of gender, race/ethnicity, protected veteran status, disability, or other protected group status.