EMB – Embryology

Lab Technician-Full Time

Department: EMB – Embryology

Salary: Based on experience

Location: Baltimore, MD

The Ludington Lab seeks an able and motivated career technician to manage our gnotobiotic fruit fly work and maintain organization of the lab and be a part of the lab ethos. Candidates should be able to design and carry out laboratory experiments, working independently and effectively while managing and prioritizing multiple projects. This position requires a thorough understanding and vigilant implementation of the scientific method, which will be required to accomplish assigned projects.

Job Duties

  • Maintain germ-free and gnotobiotic fly stocks for the lab.
  • Derive germ-free flies and inoculate them with specific bacteria (which makes the gnotobiotic).
  • Keep track of media supplies and coordinate orders for the lab to maintain ample supplies. This will require daily to weekly coordination with the part-time media technician.
  • Fly breeding, sort unmated females, perform defined crosses, genotyping.
  • Fly phenotyping, such as lifespan, fecundity, development time, automated behavior assays, dissecting gut tissues and preparing them for downstream analyses including fixing and staining for microscopy.
  • Perform basic molecular biology experiments such as, but not limited to, molecular cloning, PCR, making competent cells, bacterial transformation, DNA miniprep, DNA plasmid prep, restriction enzyme digest, running and analyzing agarose gel data to determine results.
  • Basic microbiology culture, including freeze cells, pull cells from -80˚C freezer stocks, culture cells on agar or liquid media, measure OD, plate to count CFUs, perform dilution series.
  • Use a liquid handling robot to make defined combinations of bacterial strains to inoculate flies.
  • Maintain and monitor shared resources, i.e. lab stocks such as plasmids, antibodies, and chemicals.
  • Make general shared lab stock solutions, including making aliquots and store them in an organized manner.
  • Perform literature searches to find necessary information for projects, e.g. recipes and protocols.
  • Attend and participate in lab meetings.
  • Work efficiently and cooperatively with other researchers in support of lab goals and projects.
  • Organize and lead lab supply purchases
  • Maintain the cleanliness of common lab space
  • Participate in rotation based lab duty.
  • Maintain precise records of work.
  • Other duties as assigned.

Please note: training will be provided as needed

Qualifications:

Bachelor degree in relevant science area and two years relevant lab experience – Or Master degree and one year relevant lab experience.

Knowledge and practice of basic laboratory biology techniques, and ability to learn, adapt, and carry out established written protocols independently.

Strong organizational and computer skills, meticulous attention to detail.

Excellent verbal and written communication skills

Enthusiasm for developing new skills and expertise as needed to achieve lab goals.

Ability to work independently and effectively.

Additional information:

The Department of Embryology is one of six departments comprising the Carnegie Institution for Science. The department functions as a private, non-profit research institute affiliated with the Johns Hopkins University and is located adjacent to the JHU Homewood campus in Baltimore, MD. The department consists of 10 laboratories studying a variety of questions in cell and developmental biology. For more information about the Department, visit https://emb.carnegiescience.edu/

Carnegie is an equal opportunity employer. All qualified applicants will receive consideration for employment and will not be discriminated against on the basis of gender, race/ethnicity, protected veteran status, disability, or other protected group status.

Please submit a copy of your resume or CV with the application.

 

Lab Technician-Part Time

Department: EMB – Embryology

Salary: Based on experience

Location: Baltimore, MD

The Ludington lab seeks a part-time media preparation technician (10-12 hours per week) to make fruit fly food, bacterial agar plates, and liquid bacterial culture broth. This role provides fundamental support to our gnotobiotic fruit fly work. The technician will be a part of the lab and may receive training to take on more responsibilities. Candidates should be highly experienced in sterile technique.

Job Duties:

  • Prepare fruit fly food under sterile conditions using an autoclave, filter sterilization, and a tissue culture hood.
  • Prepare bacterial agar plates under sterile conditions using an autoclave, filter sterilization, and a MediaJet.
  • Keep track of media supplies and coordinate orders for the lab to maintain ample supplies. This will require daily to weekly coordination with the lab manager.
  • Maintain precise records of work.
  • Communicate with lab manger about the needs of the lab to plan work.
  • Other assigned duties where training will be provided.

Other Responsibilities:

Organize assigned projects within a daily schedule to ensure goals are met. Meet routinely with supervisor. Report work issues to supervisor promptly to assist resolution.

Qualifications:

  • Bachelor degree in relevant science area and 6 months of relevant lab experience.
  • Knowledge and practice of sterile technique and other basic laboratory biology practice.
  • Ability to follow established written protocols independently after training.
  • Ability to calculate recipes for chemical solutions.
  • Meticulous attention to detail.Ability to work independently and effectively
  • Ability to work independently and effectively.
  • Maintain the fly room, bacteria culture area, sink area, chemical shelves and chemical weigh in area.

Additional information:

The Department of Embryology is one of six departments comprising the Carnegie Institution for Science. The department functions as a private, non-profit research institute affiliated with the Johns Hopkins University and is located adjacent to the JHU Homewood campus in Baltimore, MD. The department consists of 10 laboratories studying a variety of questions in cell and developmental biology. For more information about the Department, visit https://emb.carnegiescience.edu/

Carnegie is an equal opportunity employer. All qualified applicants will receive consideration for employment and will not be discriminated against on the basis of gender, race/ethnicity, protected veteran status, disability, or other protected group status.

Please submit a copy of your resume or CV with the application. 

 

Research Associate-Fan Lab

Department: EMB – Embryology

Salary: Based on experience

Location: Baltimore, MD

Job Summary:

A research associate position to study muscle regeneration and muscular dystrophy using the mouse model is available in the laboratory of Dr. Chen-Ming Fan at the Carnegie Institution for Science, Department of Embryology, in Baltimore, MD.

The research objective is to understand normal and pathological muscle biology from the stem cell perspective.  The position requires a highly-motivated applicant with experience in stem cell biology. The position involves conducting research using mouse as a model organism. Applicants are expected to have a Ph.D. with hands-on experimental expertise in molecular biology, cell biology, and genetics. The primary role of this position is to conduct research projects funded by the NIH both independently as well as in a team effort to study muscle stem cell quiescence, activation, differentiation, in normal, aging and diseased conditions.

The Carnegie Department of Embryology follows the current NIH-NRSA postdoctoral stipend scale. Our postdoctoral benefit package includes health care, sick leave, annual leave, parental leave, life insurance, and paid holidays.

Qualifications:

An ideal candidate will be creative, industrious, have excellent communication skills, and enjoy working independently as well as collaboratively. A Ph.D. in a related field and fluency in spoken and written English is required for the position.

To apply:

A CV, statement of research interest, and contact information for three references are required to apply.

Additional information:

The Department of Embryology is one of six departments comprising the Carnegie Institution for Science. The department functions as a private, non-profit research institute affiliated with the Johns Hopkins University and is located adjacent to the JHU Homewood campus in Baltimore, MD. The department consists of 10 laboratories studying a variety of questions in cell and developmental biology. For more information about the Department, visit https://emb.carnegiescience.edu/

Carnegie is an equal opportunity employer. All qualified applicants will receive consideration for employment and will not be discriminated against on the basis of gender, race/ethnicity, protected veteran status, disability, or other protected group status.

 

Postdoctoral Researcher – Farber Lab

Department: EMB – Embryology

Salary: NIH-NRSA Stipend Scale

Location: Baltimore, MD

Job Summary:

A postdoctoral position to identify small molecules and genetic pathways that regulate lipid metabolism is available immediately in the laboratory of Dr. Steven Farber at the Carnegie Institution for Science, Department of Embryology, in Baltimore, MD.

The position requires a highly-motivated applicant with a strong interest in questions of lipid metabolism. The position involves conducting a state-of-the-art pharmacogenetic screen for modifiers of lipoprotein metabolism and studies at the cellular and subcellular levels to characterize zebrafish with mutations in genes affecting digestive organ lipid uptake, metabolism, transport and storage.

The Farber laboratory performs studies at the nexus of cell biology, metabolism and digestive physiology.  The Carnegie Department of Embryology follows the current NIH-NRSA postdoctoral stipend scale and our postdoctoral benefit package includes health care, sick leave, annual leave, parental leave, life insurance, and paid holidays.

Qualifications:

An ideal candidate will be creative, industrious, have excellent communication skills, and enjoy working independently as well as collaboratively. A Ph.D. in a related field and fluency in spoken and written English is required for the position.

To apply:

A CV, statement of research interest, and contact information for three references are required to apply.

Additional information:

The Department of Embryology is one of six departments comprising the Carnegie Institution for Science. The department functions as a private, non-profit research institute affiliated with the Johns Hopkins University and is located adjacent to the JHU Homewood campus in Baltimore, MD. The department consists of 10 laboratories studying a variety of questions in cell and developmental biology. For more information about the Department, visit https://emb.carnegiescience.edu/

Carnegie is an equal opportunity employer. All qualified applicants will receive consideration for employment and will not be discriminated against on the basis of gender, race/ethnicity, protected veteran status, disability, or other protected group status.