EMB – Embryology

Laboratory Technician- Ludington Lab

Department: EMB – Embryology

Salary: Based on experience

Location: Baltimore, MD

Job Summary:

The Ludington lab seeks an energetic Lab Technician to join our team studying the gut microbiome in fruit flies.  The position will perform experiments using germ-free fruit flies and flies associated with defined gut bacterial flora (gnotobiotic). We’re looking for someone to support researchers in the lab by conducting experiments according to standard operating protocols and maintaining reagents (such as making germ-free fly food and bacteria media). But fear not – we have robots to assist you! The technician must work independently and effectively while managing and prioritizing multiple projects. This position requires a thorough understanding and vigilant implementation of the scientific method, which will be required to accomplish assigned projects. Experience with science and sterile technique is required, but we’ll teach you everything you need to know about germ-free flies. In the past, we’ve found that people with commercial kitchen experience picked up the skills quickly. Paranoia about germs is key!

To learn more about the Ludington Lab click here.

Job Duties:

  • Maintain germ-free and gnotobiotic fly stocks for the lab.
  • Derive germ-free flies and inoculate them with specific bacteria (which makes them gnotobiotic).
  • Keep track of media supplies and coordinate orders for the lab to maintain ample supplies. This will require daily to weekly coordination with the part-time media technician.
  • Fly breeding, sort unmated females, perform defined crosses, genotyping.
  • Fly phenotyping, such as lifespan, fecundity, development time, automated behavior assays, dissecting gut tissues and preparing them for downstream analyses including fixing and staining for microscopy.
  • Perform basic molecular biology experiments such as, but not limited to, molecular cloning, PCR, making competent cells, bacterial transformation, DNA miniprep, DNA plasmid prep, restriction enzyme digest, running and analyzing agarose gel data to determine results.
  • Basic microbiology culture, including freeze cells, pull cells from -80˚C freezer stocks, culture cells on agar or liquid media, measure OD, plate to count CFUs, perform dilution series.
  • Use a liquid handling robot to make defined combinations of bacterial strains to inoculate flies.
  • Maintain and monitor shared resources, i.e. lab stocks such as plasmids, antibodies, and chemicals.
  • Make general shared lab stock solutions, including making aliquots and store them in an organized manner.
  • Attend and participate in lab meetings.
  • Work efficiently and cooperatively with other researchers in support of lab goals and projects.
  • Organize and lead lab supply purchases
  • Maintain the cleanliness of common lab space
  • Participate in rotation based lab duties, such as cleaning incubators, chemical inventory, etc.
  • Maintain precise records of work.
  • Other duties as assigned.

Other:

  • Occasional evening or weekend work may be required.
  • Please note this job description is not designed to cover or contain a comprehensive listing of activities, duties or responsibilities that are required of the employee for this position. Duties, responsibilities and activities may change over time.

Qualifications:

  • Bachelor degree in relevant science area.
  • Knowledge and practice of basic laboratory biology techniques, and ability to learn, adapt, and carry out established written protocols independently.
  • Strong organizational and computer skills, meticulous attention to detail.
  • Excellent verbal and written communication skills
  • Enthusiasm for developing new skills and expertise as needed to achieve lab goals.
  • Ability to work independently and effectively.

Additional information:

The Department of Embryology is one of six departments comprising the Carnegie Institution for Science. The department functions as a private, non-profit research institute affiliated with the Johns Hopkins University and is located adjacent to the JHU Homewood campus in Baltimore, MD. The department consists of 10 laboratories studying a variety of questions in cell and developmental biology. For more information about the Department, visit https://emb.carnegiescience.edu/

Carnegie is an equal opportunity employer. All qualified applicants will receive consideration for employment and will not be discriminated against on the basis of gender, race/ethnicity, protected veteran status, disability, or other protected group status.

Research Scientist/Lab Manager

Department: EMB – Embryology

Salary: Based on experience

Location: Baltimore, MD

Essential Functions:

  • The candidate will be responsible for the design and implementation of one or more independent projects. With minimal supervision, determine most suitable methods to be used in independent research projects; perform scientific experiments and procedures; interpret results and determine consistency with experimental goals; review literature; interpret subsequent data. Often explores new areas and seeks opportunities to apply new knowledge to work. Train and manage lab technician to support research projects. The exercise of judgement is a prime factor in the performance of duties. Incumbent is vested with final authority to make decisions within the limits of established policies and practices.

Principle Responsibilities:

  • Independently perform laboratory research activities related to assigned project
  • Analyze data, formulate conclusions and create appropriate statistical presentation, including graphs and charts, and prepare and present written or oral progress reports regarding studies
  • Administer day-to-day laboratory operations including monitoring budget, ensuring quality control and safety compliance, development policies and procedures and ordering supplies
  • Maintain proper operation of research equipment, ensuring proper operation records, cleaning, ordering of required components and resolving technical issues
  • Actively contributes to laboratory meetings and planning sessions
  • Prepare written reports and manuscripts for submission to peer-reviewed journals for publication
  • Train new students, postdoctoral researchers, and technical personnel in a variety of laboratory techniques
  • Manage lab technician to support the research of students and postdoctoral researchers, as well as fulfill general laboratory needs
  • Work efficiently and cooperatively with a team of researchers in support of lab goals and projects

Organization Scope and Impact:

Job impacts immediate activities of work unit. Exercises scientific and administrative judgment and assumes responsibility for decisions, consequences and results having an impact on people, costs and/or research success.

Interactions with Others:

Organizes projects assigned within daily workload to ensure goals are met. Procedures are defined in broad terms as related to objectives, but work methods and approaches to work problems are determined entirely by the incumbent who is self-supervising because of his/her extensive technical and specialized knowledge and/or experience. Reports any work and management issues to supervisor promptly to assist resolution. Participates in group problem solving. Explains basic and routine procedures to group members and trains group members as requested.

Other:

  • Occasional evening or weekend work may be required.
  • Please note this description is not designed to cover or contain a comprehensive listing of activities, duties or responsibilities that are required of the employee for this position. Duties, responsibilities and activities may change at any time with or without notice.

Qualifications:

  • PhD degree in a relevant field and one or more positive letters of reference from scientist(s) familiar with the candidate’s research and work ethic
  • Experience in the development and use of mouse models.
  • Able to critically review information and understand new concepts and techniques with minimal assistance
  • Strong organizational and computer skills, meticulous attention to detail
  • Excellent verbal and written communication skills
  • Enthusiasm for developing new skills and expertise
  • Ability to work independently and effectively

Physical Requirements:

  • Occasionally lifts and /or carries objects weighing up to 25 pounds
  • Occasionally stoops and bends
  • Occasionally reaches and grasps with arms and hands above head
  • Work is performed in a normal laboratory environment where there are relatively few physical discomforts due to dust, dirt, noise, or similar
  • Work involves possible exposure to malodorous vapors, low-dose radiation, contamination by toxic chemical and acids and presence of carcinogenic substances

Additional Information:

The Department of Embryology is one of six departments comprising the Carnegie Institution for Science. The department functions as a private, non-profit research institute affiliated with the Johns Hopkins University and is located adjacent to the JHU Homewood campus in Baltimore, MD. The department consists of 10 laboratories studying a variety of questions in cell and developmental biology. For more information about the Department, visit https://emb.carnegiescience.edu/

Carnegie is an equal opportunity employer. All qualified applicants will receive consideration for employment and will not be discriminated against on the basis of gender, race/ethnicity, protected veteran status, disability, or other protected group status.

Aquatic Facility Technician

Department: EMB – Embryology

Salary: Based on experience

Location: Baltimore, MD

Aquatic Facility Technician

Job Summary:

Feeding of fish, filter changes, regular and scheduled maintenance, troubleshooting, assessing health of stocks and water quality, etc. Train lab members and student helpers in matters related to zebrafish husbandry and facility maintenance, and make them aware of changes in protocols.  Maintain cleanliness, usability and productivity of the facility.

Essential Duties:

  1. Prepare and harvest food.
  2. Feed all fish a variety of stage-appropriate foods at regularly scheduled intervals.
  3. Inspect fish aquaria and change tanks as required to maintain a healthy environment for the animals.
  4. Monitor and maintain water quality.
  5. Keep daily logs of facility conditions.
  6. Observe fish daily and prepare routine reports on the health, death and census of animals.
  7. Be able to troubleshoot problems when they arise and contact building manager if problem cannot be immediately remedied.
  8. Perform system maintenance on a day-to-day basis.
  9. Master the use of and perform troubleshooting on facility databases and computers.
  10. Order supplies for the facility.
  11. Train and advise new lab members in feeding and daily maintenance protocols for consistency in care.
  12. Make suggestions for improvements to enhance care of animals and optimize use of time.
  13. Keep labs aware of any changes or issues in the facility, maintain open communication.
  14. Provide consistent care and keep facility clean, well-organized and usable for lab members.
  15. Because this position involves live animals that require a very controlled for their viability, the facilities manager must be available to respond at any time of the day and on weekends to automated telephone alerts generated when fish facility parameters deviate from accepted norms. If it is not possible to respond to a given alarm in a timely fashion, it is expected that that they call the PIs and/or lab members.

Organizational Scope and Impact of Position

Job has an impact on the immediate research activities of the laboratories. Problems must be addressed effectively and reported in a timely manner.  On-going communication with members of research labs and PI’s is also essential. 

Interactions with Others:

Expected to interact productively with and assist laboratory members when necessary. Collaboration and frequent communication with the Facilities Department is expected.

Preferred Qualifications

Education and Experience:

B.S. degree preferred and 1 year of equivalent work experience in a comparable animal care facility.

Physical Requirements:

  • Routinely lifts and/or carries objects weighing up to 50 pounds.
  • Exposed regularly to some exceptional conditions such as warm temperatures and long periods of standing or squatting.

Additional Information:

The Department of Embryology is one of six departments comprising the Carnegie Institution for Science. The department functions as a private, non-profit research institute affiliated with the Johns Hopkins University and is located adjacent to the JHU Homewood campus in Baltimore, MD. The department consists of 10 laboratories studying a variety of questions in cell and developmental biology. For more information about the Department, visit https://emb.carnegiescience.edu/

Carnegie is an equal opportunity employer. All qualified applicants will receive consideration for employment and will not be discriminated against on the basis of gender, race/ethnicity, protected veteran status, disability, or other protected group status.

 

 

Postdoctoral Researcher- Fan Lab

Department: EMB – Embryology

Salary: NIH-NRSA Postdoctoral Stipend Scale

Location: Baltimore, MD

Job: Postdoctoral Researcher – Fan Lab

Department: Embryology

Salary: NIH-NRSA Stipend Scale

Location: Baltimore, MD

Job Summary:

A postdoctoral position to study muscle regeneration and muscular dystrophy using the mouse model is available in the laboratory of Dr. Fan at the Carnegie Institution for Science, Department of Embryology, in Baltimore, MD.

The research objective is to understand normal and pathological muscle biology from the stem cell perspective.  The position requires a highly-motivated applicant with a strong interest in stem cell biology. The position involves conducting research using mouse as a model organism. Applicants are expected to have research background in molecular biology, cell biology, and genetics. The research approach is to use up-to-date methods and develop new techniques and tools to study muscle stem cell quiescence, activation, differentiation, in normal, aging and diseased conditions.

The Carnegie Department of Embryology follows the current NIH-NRSA postdoctoral stipend scale and our postdoctoral benefit package includes health care, sick leave, annual leave, parental leave, life insurance, and paid holidays.

To learn more about the Fan Lab please click here.

Qualifications:

An ideal candidate will be creative, industrious, have excellent communication skills, and enjoy working independently as well as collaboratively. A Ph.D. in a related field and fluency in spoken and written English is required for the position.

To apply:

A CV, statement of research interest, and contact information for three references are required to apply.

Additional information:

The Department of Embryology is one of six departments comprising the Carnegie Institution for Science. The department functions as a private, non-profit research institute affiliated with the Johns Hopkins University and is located adjacent to the JHU Homewood campus in Baltimore, MD. The department consists of 10 laboratories studying a variety of questions in cell and developmental biology. For more information about the Department, visit https://emb.carnegiescience.edu/

Carnegie is an equal opportunity employer. All qualified applicants will receive consideration for employment and will not be discriminated against on the basis of gender, race/ethnicity, protected veteran status, disability, or other protected group status.