EMB – Embryology

Research Technician

Department: EMB – Embryology

Salary: TBD

Location: Baltimore, MD

Job Summary:

The Kostova laboratory at Carnegie’s Department of Embryology in Baltimore is searching for a full-time research technician. This is a great opportunity to work with a postdoctoral fellow using cutting-edge technologies to answer fundamental questions of cell biology. Ideal candidates should have a B.S. in Biology or a related field, experience in modern molecular biology techniques, a strong work ethic, and be able to join the position for at least 2 years.

We are investigating the fundamental question of how different types of ribosomes regulate translation and embryogenesis. Despite ribosomes’ importance, they are traditionally seen with minimal regulatory activity. Exciting new studies challenge this concept and provide evidence that organisms assemble ribosomes with different makeups during development in response to stress, including the development of multiple cancers. Although ribosome heterogeneity is hypothesized to alter translation, little experimental data exist to demonstrate this. The zebrafish embryo provides a power system to tackle this question. We use cutting-edge technology, such as genome editing and high throughput sequencing technology, to tag different ribosome populations and determine their ability to synthesize proteins.

We are looking for a highly motivated individual with strong organizational skills to join our team and help explore these novel areas of biology.

Key Responsibilities:

  • Perform genome editing and characterize zebrafish strains (genotyping, qPCR, Western blotting)
  • Isolation of ribosomes and RNA analysis (sucrose gradient fractionation, immunoprecipitation, RNA purification, and electrophoresis)
  • Maintenance and upkeep of zebrafish-related infrastructure

Requirements:

  • B.S. in Biology (or equivalent)
  • Lab experience
  • Strong interpersonal skills and ability to work as part of a team

Preferred Skills:

  • Modern molecular biology techniques, e.g., DNA/RNA purification, cDNA synthesis, qPCR, plasmid cloning, SDS­PAGE, and Western blotting
  • Some experience working with zebrafish or other vertebrates in a research laboratory.
  • Great organizational skills
  • Excellent written and oral communication skills

If interested please send the following documents to kostova@carnegiescience.edu:

  • Cover letter
  • Curriculum Vitae
  • Academic transcript
  • Recommendation letters (at least 2)

Research Environment:

The Department of Embryology is one of six departments comprising the Carnegie Institution for Science. The department functions as a private, non-profit research institute affiliated with Johns Hopkins University and is located adjacent to the JHU Homewood campus in Baltimore, MD. The Department consists of 10 laboratories studying a variety of questions in cell and developmental biology. For more information about the Department, visit https://emb.carnegiescience.edu/.

Additional Information:

Carnegie is an equal opportunity employer. All applicants will receive consideration for employment without regard to race, color, religion, gender, sexual orientation, gender identity or expression, national origin, age, genetic information, disability, veteran status, or any other characteristics protected by law.

At Carnegie, we are committed to building a diverse and inclusive community. We believe academic environments should be places where diverse groups of people with various viewpoints and ideas can thrive and work together. As such, we encourage applicants from under-represented groups and backgrounds to apply.

 

Electron Microscopy Research Assistant

Department: EMB – Embryology

Salary: TBD

Location: Baltimore, MD

The Department of Embryology of the Carnegie Institution for Science is recruiting an Electron Microscopy (EM) Research Assistant to support the EM research of the Department. This includes but is not limited to research on animal and plant tissues and microbes for examination by transmission electron microscopy (TEM) using a wide range of basic and advanced EM technical procedures.

This individual will assist the current EM Specialist by conducting EM sample processing, TEM operation, and lab management including ordering, data keeping, reagent preparation, instrument maintenance, and training users on TEM operation as well as operation of another accessory instrument. This position will also supervise daily lab usage in the absence of the EM Specialist.

Responsibilities

  • Perform room temperature resin embedding of various plant, animal tissues, cells and microbes
  • Perform ultramicrotomy using diamond knives
  • Perform negative staining of microbes, purified organelles, and macromolecular complexes
  • Perform advanced techniques including but not limited to immunoelectron microscopy, high-pressure freezing and freeze substitution (HPF/FS), and microwave processing
  • Maintain records of all processed specimens including a detailed account of all steps used in processing each specimen
  • Manage the storage and disposal of biohazardous reagents
  • Perform general maintenance (i.e. replace electron gun filament) and troubleshoot basic problems of electron microscopes and ancillary equipment. Open/close and supervise the lab in the absence of the EM Specialist
  • Perform image post-processing and analysis of results

Requirements

BA/BS in biology, chemistry, or related scientific field with hands-on experience in basic laboratory and scientific methods. Knowledge and skills in TEM sample processing and instrument operation is preferred but not required.  Research-based experience preferred. Consideration will be given to unique combinations of education and experience. Individuals with an advanced degree are encouraged to apply. Salary will be commensurate with education and experience.

Other:          

May require training related to occupational safety and health, environmental compliance, shipping of hazardous materials, and/or ionizing radiation.

Research Environment:

The Department of Embryology is one of six departments comprising the Carnegie Institution for Science. The department functions as a private, non-profit research institute affiliated with Johns Hopkins University and is located adjacent to the JHU Homewood campus in Baltimore, MD. The Department consists of 10 laboratories studying a variety of questions in cell and developmental biology. For more information about the Department, visit https://emb.carnegiescience.edu/.

Carnegie is an equal opportunity employer. All applicants will receive consideration for employment without regard to race, color, religion, gender, sexual orientation, gender identity or expression, national origin, age, genetic information, disability, veteran status, or any other characteristics protected by law.