Department: EMB – Embryology
Location: Baltimore, MD
The Ludington Lab is seeking a Lab Manager who is able to work independently and effectively while managing and prioritizing multiple projects and communicating effectively to enhance the cohesiveness of the lab. An individual in this position will be responsible for maintaining supply stocks, preparing and managing the research materials including growth media, and maintaining the laboratory stocks of model organisms. Responsibilities will also include assisting other lab members in research projects involving germ-free and gnotobiotic Drosophila and providing materials to assist their work. This position requires a thorough understanding and vigilant implementation of the scientific method, which will be required to accomplish assigned projects. Experience with science and sterile technique is required. Paranoia about germs is key.
This position presents a unique opportunity for a recent graduate looking to gain skills and experience in a laboratory environment.
Essential Job Duties:
- Maintain germ-free and gnotobiotic fly stocks for the lab.
- Fly phenotyping, such as lifespan, fecundity, development time, automated behavior assays, dissecting gut tissues, and preparing them for downstream analyses including fixing and staining for microscopy.
- Perform basic molecular biology experiments such as, but not limited to, molecular cloning, PCR, making competent cells, bacterial transformation, DNA miniprep, DNA plasmid prep, restriction enzyme digest, and running and analyzing agarose gel data to determine results.
- Keep track of media supplies and maintain ample supplies. Media includes fly media and bacterial media. This task requires daily to weekly coordination with other lab members to assess and meet their needs.
- Order and maintain core lab supplies including lab reagents and consumables.
- Derive germ-free flies and inoculate them with specific bacteria (which makes them gnotobiotic).
- Fly breeding, sorting unmated females, performing defined crosses, and genotyping.
- Attend and participate in lab meetings.
- Make generally shared lab stock solutions, including making aliquots, and store them in an organized manner.
- Maintain precise records of work.
- Other duties as assigned.
- Basic microbiology culture, including freeze cells, pull cells from -80˚C freezer stocks, culture cells on agar or liquid media, measure OD, and plate count CFUs.
- Prepare and send strains to collaborators.
- Maintain and monitor shared resources, i.e. lab stocks such as plasmids, antibodies, and chemical reagents.
- Occasional evening or weekend work may be required.
- Work efficiently and cooperatively with other researchers in support of lab goals and projects.
- Maintain lab equipment.
- Participate in rotation-based lab duties, such as cleaning incubators, chemical inventory, etc.
- Maintain the cleanliness of the common lab space.
- Please note this job description is not designed to cover or contain a comprehensive listing of activities, duties, or responsibilities that are required of the employee for this position. Duties, responsibilities, and activities may change over time.
Desired Education and Skills:
- Bachelor’s degree in the relevant science area.
- Knowledge and practice of basic laboratory biology techniques, and ability to learn, adapt and carry out established written protocols independently.
- Strong organizational and computer skills, and meticulous attention to detail.
- Excellent verbal and written communication skills.
- Enthusiasm for developing new skills and expertise as needed to achieve lab goals.
- Ability to work independently and effectively.
At Carnegie, we are committed to building a diverse and inclusive community. We believe academic environments should be places where diverse groups of people with various viewpoints and ideas can thrive and work together. As such, we encourage applicants from under-represented groups and backgrounds to apply.
Carnegie is an equal-opportunity employer. All qualified applicants will receive consideration for employment and will not be discriminated against on the basis of gender, race/ethnicity, protected veteran status, disability, or another protected group status.