Department: DPB – Plant Biology
Location: Stanford, CA
A Postdoctoral Fellow position is available to analyze maize anther proteomes, jointly sponsored by Dr. Virginia Walbot (Stanford University) and Dr. Shouling Xu (Department of Plant Biology, Carnegie Institution for Science. The position is located on the Stanford CA. Using fully fertile and male-sterile, anther defective mutants already characterized by microscopy and single cell RNA-seq, learn diverse proteomics techniques to monitor protein abundances, de novo synthesis, and post-translational modifications in developing anthers to answer fundamental questions in developmental biology.
Nan, G-L., et al. 2022. Cascades of bHLH-regulated pathways program maize anther development. Plant Cell 34: 1207-1225. doi: org/10.1093/plcell/koac007 Highlight M. Osnato. 2022. Fantastic four: bHLH factors and the making of pollen. Plant Cell 34: 1151-1152. doi.org/10.1093/plcell/koac028
Nelms, B. and V. Walbot. 2022. Gametophyte genome activation occurs at pollen mitosis I in maize. Science 375: 284-289, plus the cover. doi: 10.1126/science.abl7392
Bi, Y. et al. 2021. Arabidopsis ACINUS is O-glycosylated and regulates transcription and alternative splicing of regulators of reproductive transitions. 2021. Nat Commun.12: 945. doi.org/10.1038/s41467-021-20929-7
Reyes, A. V. et al. 2022. Application of parallel reaction monitoring in 15N labeled samples for quantification. Front. Plant Sci., doi.org/10.3389/fpls.2022.832585
We prefer candidates with experience in either maize genetics/molecular biology or proteomics to work with the Walbot lab on carefully staged anthers and specific anther cell types. Candidates will perform MS work at Carnegie Mass Spec facility (on the Stanford campus) directed by Dr. Shouling Xu. The facility is currently equipped with Orbitrap Q-Exactive and is expected to have additional Orbitrap Eclipse with ETD in the fall. The facility is equipped with three HPLC/FPLC instruments for offline fractionations. Position to start in summer or fall 2022. Please click the Apply Now box below and send your CV, a cover letter, along with the names of 3 references.
Additional information: The Department of Plant Biology of Carnegie Institution is located on the campus of Stanford University. Formerly known as the Carnegie Institution of Washington, the Carnegie Institution for Science is a U.S.-based non-profit, privately endowed, research institution. Andrew Carnegie founded the Carnegie Institution of Washington in 1902 as an organization for scientific discovery to serve as a home to exceptional individuals capable of working at the cutting edge of their research fields. Carnegie investigators are leaders in the fields of plant biology, developmental biology, earth and planetary sciences, astronomy, and global ecology. The Department of Plant Biology has state-of-the-art facilities for microscopy/imaging and mass spectrometry, laboratory facilities for molecular genetic studies of plants, growth facilities for photosynthetic microbes and higher plants, field experimental stations, and high-performance computing nodes.
Carnegie is an equal opportunity employer. All applicants will receive consideration of employment without regard to race, color, religion, gender, sexual orientation, gender identity or expression, national origin, age, genetic information, disability, veteran status or any other characteristics protected by law. We are committed to building an inclusive and diverse research community and we encourage applications by members of all underrepresented groups.